Used HPLC column Tosoh TSKgel Boronate-5PW 7.5mm x 7.5 cm 10 um 13066

Used HPLC column Tosoh TSKgel Boronate-5PW 7.5mm x 7.5 cm 10 um. cat no 13066 Lot gar4thmixTSKgel Boronate-5PW is based on a 100 nm pore size polymethacrylate base material bonded with m-aminophenyl boronate. This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates. glycosylated proteins. and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers. such as HEPES and morpholine. while desorption takes place in carbohydrate or amine-containing mobile phaselike sorbitol or Tris. Applications include nucleic acids. catecholamines. nucleosides. and nucleotides.